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KMID : 0811720100140050311
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Korean Journal of Physiology & Pharmacology
2010 Volume.14 No. 5 p.311 ~ p.316
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P2X and P2Y Receptors Mediate Contraction Induced by Electrical Field Stimulation in Feline Esophageal Smooth Muscle
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Cho Young-Rae
Jang Hyeon-Soon
Kim Won
Park Sun-Young
Sohn Uy-Dong
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Abstract
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It is well-known that electrical field stimulation (EFS)-induced contraction is mediated by a cholinergic mechanism and other neurotransmitters. NO, ATP, calcitonin gene-related peptide (CGRP), and substance P are released by EFS. To investigate the purinergic mechanism involved in the EFS-induced contraction, purinegic receptors antagonists were used. Suramine, a non-selective P2 receptor antagonist, reduced the contraction induced by EFS. NF023 (10?7∼10?4 M), a selective P2X antagonist, inhibited the contraction evoked by EFS. Reactive blue (10?6∼10?4 M), selective P2Y antagonist, also blocked the contraction in a dose-dependent manner. In addition, P2X agonist ?,?-methylene 5’-adenosine triphosphate (??MeATP, 10?7∼10?5 M) potentiated EFS-induced contraction in a dose-dependent manner. P2Y agonist adenosine 5’-[?-thio]diphosphate trilithium salt (ADP?S, 10?7∼10?5 M) also potentiated EFS-induced contractions in a dose-dependent manner. Ecto-ATPase activator apyrase (5 and 10 U/ml) reduced EFS-induced contractions. Inversely, 6-N,N-diethyl-D-?,?- dibromomethylene 5’-triphosphate triammonium (ARL 67156, 10?4 M) increased EFS-induced contraction. These data suggest that endogenous ATP plays a role in EFS-induced contractions which are mediated through both P2X-receptors and P2Y-receptors stimulation in cat esophageal smooth muscle.
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KEYWORD
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Electrical field stimulation, Smooth muscle, G protein, P2 receptor, ATP, Calcium
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